Acs Exam Latest Exam Session Newest Exam Guaranteed Pass Graded A+

sequencing {Ans: - uses ddNTP: ddNTP does not have OH (deoxy) on C2/C3 (dideoxy) - DNA polymerase will stop when ddNTPs are incorporated onto the DNA strand - determines the nucleotide sequence in one strand}semi-conservative {Ans: one parent strand and one newly synthesized strand}miRNA {Ans: - micro RNA - silencing and post-transcriptional regulation of gene expression}Tm {Ans: temperature at which 50% of DNA is annealed (melting point of GC-rich DNA is higher than AT rich DNA due to number of G-C hydrogen bonds -- 3)}intro to metabolism {Ans: high energy compounds: - ATP (and its triphosphate derivatives) - acetyl-CoA - reduced cofactors: NADH, NADPH, QH2 oxidation | reduction: many metabolic pathways include oxidation-reduction reactions - CoQ is an example}REN {Ans: restriction endonuclease recombination}