Clinical Chemistry 2: Enzymes (Some Instrumentation) Questions And Accurate Answers Verified 100% 2024-2025

Clinical Chemistry 2: Enzymes (some instrumentation) questions and accurate answers verified 100% 2024-2025 What causes enzymes to be increased in serum? Increased after cellular injury. What is the active site on an enzymes structure? The active site is where the substrate reacts. What is the term for a nonprotein molecule that must bind to a particular enzyme before a reaction can occur? Co-factor (required by some enzymes not all) Are the following coenzymes or activators? - inorganic, usually ions, e.g., metals (Mg2+, Na+, K+, Ca2+) or non-metals (Cl-, Br-) Activators (Some needed for reactions; others enhance reactions)Why can't an enzyme be run on plasma from a EDTA collection tube? It can't be used because serum is required for enzyme measurement due to some anticoagulants binding Ca2+ and Mg2+, which are needed by many enzymes.(activators) What is the coenzyme for both AST and ALT? Pyridoxal phosphate What are 2 interferences of ALT when measuring? Hemolysis and lipemia may interfere. What is the coenzyme of Lactate Dehydrogenase(LD)? NAD Are LD levels increased or decreased in the following disorders? (specify slight, moderate, or large change) - acute viral hepatitis and cirrhosis: - biliary tract disease: - metastatic cancer of liver: - acute viral