Given five unlabeled bottles, each bottle containing one of the following amino acids: arginine, histidine, cysteine, proline, and tryptophan. What method would be best to determine which bottle contained the tryptophan? a. Gel filtration b. Optical rotation c. Electrophoresis d. Ultraviolet spectroscopy e. Analytical ultracentrifugation {Ans: D}During the formation of glycocholate, cholic acid, CoASH, and ATP form cholyl-CoA, AMP, and pyrophosphate. What reaction intermediate would you expect in this process? A. cholyl phosphate B. cholyl adenylate C. cholyl pyrophosphate D. cholyl group bound to a protein by a thioester linkage {Ans: B}Which amino acid has an R-group pKa closest to physiological pH? A. lysine B. histidine C. glutamate D. arginine E. aspartate {Ans: B}Which amino acid has an R-group pKa closest to physiological pH? a. lysine b. glutamate c. arginine d. histidine e. aspartate {Ans: D}Which of the following would allow you to determine the isoelectric point of a protein? A. protein quaternary structure B. protein solubility as a function of pH C. protein size D. protein shape E. protein tertiary structure {Ans: B}In a Michaelis-Menten plot, the x-axis represents ________, and the y-axis represents ________. a. maximum velocity, substrate concentration b. initial velocity, substrate