Btec Applied Science Level 3 Gram Straining Study Guide Questions And Correct Answers

Why is it important that microbiologists can correctly identify bacteria that cause infections? {Ans: 1)Enable them to decide the most effective treatment}Why are colours and shape of cells important? {Ans: 1)Help's which type of bacteria are present}What happens if you see the gram negative? Why? {Ans: 1)Smear has retained the pink safranin stain shows that bacteria is present. 2)The thinner cell walls and lipid membranes allow ethanol (applied to the method) to wash off all the crystal violet purple stain and to then retain the pink safranin stain.}Briefly explain how to carry out gram staining. {Ans: 1)Prepare a heat fixed bacterial smear 2)Cover the smear with crystal violet for 60 seconds then rinse with water 3)Cover the smear with gram iodine for 30 seconds then rinse with water 4)Briefly decolourise the smear with acetone alcohol then rinse with water 5)Cover the smear with saffranin for 60 seconds then rinse with water and dry 6)Observe the dried smear with the microscope using oil immersion}What happpens if you see the gram positive? {Ans: 1)Purple stain when observing the smear under a microscope showing that it's present bacteria}What happens during the staining technique? {Ans: 1)Two stains are