Cht Exam Complete Study Guide Questions With Correct Answers

CHT EXAM COMPLETE STUDY GUIDE QUESTIONS WITH CORRECT ANSWERS.It is possible to convert the plasmainto serum by recalcifying the plasma and allowing coagulation to occur. Answer- Calcium is added to the plasma to form a complex with the citrate present in the anti-coagulant. This restores a normalionized calcium level allowing clotting to occur.Polyspecific antiserumcan be______ with lymphocytes to determine if there is a single antibody or multipleantibodies. Answer- absorbedAnother application of lymphocyteabsorption is to use the patient's own cells to remove_____. Absorption can be performedat cold or warm temperatures depending on the type of antibody. Answer- autoantibody_________ are typicallycold reacting (4oC) and________are typically warm reacting (22-37oC). Answer- Autoantibodies B cell antibodiesPatient serum may contain autoantibodies and/or IgM antibodies that may interfere the binding ofanti-HLA IgG antibodies to the antigen. In complement dependent assays (CDC), IgM antibodiescan activate complement; potentially leading to ___________. Answer- false positive reactivity.DTT Answer- dithiothreitol, also known as Cleland's ReagentDTT inactivates IgM molecules by cleaving the _________ in thepentamer structure. Answer- disulphide bondsHeat inactivation works by cleaving the disulphide bonds in thepentamer structure, but is less specific than _____. Answer- DTT.ALA Answer- therapeutic antilymphocyteantibodiesThymoglobulin Answer- purified, pasteurized, gamma immune globulin,obtained by immunization of rabbits with human thymocytesOKT3