Biol 2051 Micro Exam 2 -General Microbiology - Lsue

This study source was downloaded by 100000859819779 from CourseHero.com on 02-15-2023 10:52:08 GMT -06:00https://www.coursehero.com/file/189639918/micro-exam-2docxpdf/ M ic ro e x am 21.Polymerase chain reaction (PCR) copies an individual gene segment into vitro with a(n)_______ primer(s). (Hint: think of a mechanism of DNA replication)A pair (two types) of DNA primers2.A(n) _____ gene is a gene that encodes a protein that is easy to detect and assay , whengenetic engineering protocol is being followed. For example, the GFP gene or LacZ geneReporter3.When a bacterium is transferred from a nutrient-rich to a nutrient-poor environment, itstarts producing ______, and ______ regulatory mechanism gets activated.Alarmones, stringent response4.All of the following are functions of heat shock proteins in bacteria EXCEPTEnhances binary fission and binds to ribosomes to enhance transcription5.When ar ginine is added to a culture already growing exponentially in a medium withoutar ginine, what occurs? (hint: think of ar ginine operon)Growth continues, but the production of enzymes required for the synthesis f ar ginineceases6.In negative control of transcription by the lac operon, how does the presence of aninducer af fect transcription?The inducer binds to the repressor , which changes its shape and makes it unable to bindto the operator sequence7.Which of the following is NOT a