Ucf Microbiology Lab Midterm Updated Exam Questions (145 Terms) With Verified Definitions Updated 2024

Which enzyme converts hydrogen peroxide to water and oxygen? {Ans: catalase}What is the primary dye used in acid-fast stain? Type? {Ans: Ziel's Carbol Fuchsin, lipid-soluble dye}EMB- Butanediol fermenters {Ans: produce less acid pale pink to lavender centers bull's-eye colonies}Urea {Ans: Urea yields 2NH3+CO2 pH indicator- phenol red pH above 8.1 positive- presence of red color negative- absence of red color Proteus- Urease+}EMB {Ans: selects for gram - differentiates lactose +/- even further Lactose + show color change Further differentiates Lactose + fermenters based on the amounts of acid produced}Acid-Fast Stain Steps & Results {Ans: 1. Cover smear with paper, and saturate with Carbol fuchsin 2. Steam while desaturating 3. Allow slide to cool 4. Decolorize with Acid Alcohol 5. Counterstain with methylene blue Results: +pink, -blue}