Csmls Chem Review Study Guide Graded A+

Absorbance equation {Ans: A = 2 - log(%T)}ISE sources of error: pH changes {Ans: - some ions very sensitive because of competitive binding to proteins in serum}membrane potentials {Ans: - membrane separates two solutions with different ionic acitivty - allows for ions to diffuse back and forth - electrical communication of two solutions w/o them mixing}most widely used stationary phase in adsorption chromatography {Ans: - polar adsorbents - L/S - silica, alumina}where would you see a fibrinogen band in electrophoresis? {Ans: - near application point- - extra, unnecessary - avoided by using serum samples}most common method for forming cations in MS {Ans: electron bombardment (electron ionization) - most commonly used in GC/MS - molecules in source bombarded with high-energy electrons to form charged molecular ions and fragments - ions formed and relative proportions are reproducible and used for qualitative identification of compound}