Chapter 19 Solutions Manual Questions And Answers 100% Verified A+ Guaranteed

*look at #43 in solutions manual* {Ans: Yee}23. What is gene therapy? {Ans: Solution: Gene therapy is the correction of a defective gene by either gene replacement or the addition of a wild-type copy of the gene. For this to work, enough of the cells of the critically affected tissues or organs must be transformed with the functional copy of the gene to restore normal physiology.}10. Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. {Ans: First, the double-stranded template DNA is denatured by high temperature. Then, primers corresponding to the ends of the DNA sequence to be amplified are annealed to the single-stranded DNA template strands. These primers are extended by a thermostable DNA polymerase so that the target DNA sequence is duplicated. These steps are repeated 30 times or more. Each cycle of denaturation, primer annealing, and extension results in doubling the number of copies of the target sequence between the primers.}37. In Figure 19.22 Bob and Joe are each homozygous for different restriction fragment patterns. How many bands would you expect to see on the gel if a person was heterozygous for the A and B patterns? Explain