Csmls Instrumentation Questions Study Guide Questions And Correct Answers

Various holes are noted in sections of paraffin-embedded liver on the flotation bath. With further ribboning, these holes decrease in size and disappear. The most likely cause of the holes is: a. overfixation b. air trapped in the tissue c. facing the block too aggresively d. infiltration with overheated paraffin {Ans: c. holes that dissapear originally occured due to agressive facing of blocks (small flecks of tissue are removed during rough facing leaving small holes)}Birefringent substances are best examined with which of the following microscopes? a. fluorescence b. electron c. light d. polarizing {Ans: d. polarizing}During cryotomy, sections of varying thickness are obtained. This can most likely be corrected by: a. lowering the cryostat temperature b. warming the blade edge c. increaing the blade tilt d. decreasing the speed of sectioning {Ans: c. too little blade tilt as well as worn or loose microtome parts}When checking the pH of a staining solution, the pH meter should be calibrated using a standard solution with a pH value: a. closest to that of the staining solution b. appoximating that of water c. in any range d. close to 7.0 {Ans: a. for the greatest accuracy, the pH meter