Energy released by the electron transport chain is used to pump H+ into which location in eukaryotic cells? {Ans: Mitochondrial inter membrane space}The active site of an enzyme is the region that: {Ans: Is involved in the catalytic reaction of the enzyme}The molecule that functions as the reducing agent (electron donor) in a redox or oxidation-reduction reaction: {Ans: Loses electrons and loses potential energy.}Why is glycolysis described as having an investment phase and a payoff phase? {Ans: It uses stored ATP and then forms a net increase in ATP.}When chemical, transport, or mechanical work is done by an organism, what happens to the heat generated? {Ans: It is lost to the environment}The primary role of oxygen in cellular respiration is to: {Ans: Act as an acceptor for electrons and hydrogen, forming water.}