Mmsc 438 Exam 2 (With Complete Solutions)

viable organisms in the blood; transient after affecting mucous membranes or infected tissues {Ans: bacteremia}Bacterial meningitis CNS infection agents in Children {Ans: S pneumon, N meningl, H influenzae tybe b}Uses the following protocols: Cells and viruses are ruptured. Nucleic acid isolation Reverse transcription on RNA target: for a strand of cDNA production (ONLY 1 amplicon produced) {Ans: Bio Film Array (PCR method)}bacterial toxins in blood stream {Ans: toxemia (endotoxins/LPS are part of the organisms, only found in GN organisms and EXOtoxins affect GP organisms and GN too)}In PCR, DNA copies (__________) = determine presence or absence of Specific gene, specific sequence (1 to 100 bp differences) {Ans: amplicon}common contaminants in collection of CSF {Ans: skin contaminants (CoNS, strep, coryn, bacillus, and cut acnes)}