Portage Learning: Microbiology, Module 3 Microscopy |questions With 100% Correct Answers | Updated & Verified | 2024

wet mount {Ans: used to observe the motility and behavior of an organism}dark field microscope {Ans: can be used to greatly increase the contrast between a specimen and background, resulting in a dark background with bright objects in it/reflects light off of the specimen at an angle/does not permit the visualization of intracellular structures}stains {Ans: can be used to examine tissues (muscle or connective), specific types of cells (blood, bacterial, etc) or even organelles within an individual cell (nucleus, ER, etc)}the ________________ the contrast between an object and its background, the harder it will be to see the object {Ans: lower}iodine {Ans: forms a stable complex, effectively trapping the dyes in the cell}heat fixation {Ans: most common method to fix a sample}before the light reaches the sample, it first passes through a ________________ converging the light beams into a focused area on the sample {Ans: condenser}pink {Ans: by counterstaining with positively charged safranin, gram-negative bacteria appears....}total magnification {Ans: objective x ocular lens=}wet mount {Ans: small liquid culture containing a microorganism of interest is prepared, added to a slide and then covered with a glass coverslip/ heat fixing is not performed}safranin