Biochemistry Data Sheet - Ph And Buffers

pH and Buffers QUESTIONS: 1. What is the pH of blood? Milk? Urine? The blood is slightly basic in nature, with a pH that ranges from 7.35 to 7.45. Pasteurized, canned, or dried milk is an acid-forming food. Because it contains lactic acid, the pH level of the milk is below normal, ranging from 6.7 to 6.9. It is mildly acidic in urine, with a normal pH ranging from 4.0 to 8, with an average value of 6.3, however the pH changes depending on the diet. 2. What is the biochemical relevance of pH? The pH is crucial in biology because it affects the structure and activity of macromolecules. Furthermore, biological processes are pH dependent; cells and organisms require a precise and consistent pH to retain their enzymes in the optimal protonation condition. Because living creatures are sensitive to pH fluctuations, they are extremely significant. More significantly, a shift in pH could be harmful. 3. Calculate the pH of the following solutions: A. 0.01M HCl Answer: pH= -log[H+] pH= -log[0.01] pH= 2 B. pH of 2.2 x 10-3 M NaOH Answer: =log12.2 103=2.66 4. Which sample exhibited buffer action? Explain. Blood is an example of a buffer action, which is