Biol 1107l Lab 5: Characterizing The Enzyme Alkaline Phosphatase

This study source was downloaded by 100000865965430 from CourseHero.com on 05-02-2023 14:59:46 GMT -05:00https://www.coursehero.com/file/198104581/Lab-5-Enzyme-Experiment-Spring-2023docx/ Lab 5: Characterizing the Enzyme Alkaline PhosphataseAPase is highly subject to degradation. To ensure its stability, it must be mixed with another protein that acts to slow its self-destruction. The protein used for this purpose is bovine serum albumin (BSA). To stabilize the APase, mix 1 ml of stock unstable APase with 4 ml of BSA . MIX THOROUGHLY invert and shake the tube holding this new solution. Record the dilution factor introduced by this step: _________5ml_____ (0.2pt) The stock APase concentration is 10 mg/ml. What is the final concentration of APase after dilution?__________2ml_______ (0.2pt)Week 1:I. Design a standard enzyme activity assay for alkaline phosphatase enzymeYou will conduct an experiment to measure the activity of APase over time. Based on your understanding of the reaction involving PNPP and APase1) (0.1pt) What is the hypothesis for this experiment? When the APase is more yellow, the activity if the enzyme is stronger and the concentration is high2) (0.1pt) What is your prediction for the results of this experiment? I predict that the APase will change color as time passes3) (0.1pt) What kinds of data will you collect? We