B C HE 4 24. E xp erim enta l B io ch em is tr y IP oly m era se C hain R eactio nP oly m era se c h ain r e actio n ( P C R) is a p ow erfu l te ch niq ue u se d in m ole cu la r b io lo gy to a m plif ys p ecif ic D NA s e quence s in a s a m ple . It w as fir s t d escrib ed b y K ary M ullis in 1 983 a nd h as s in ceb eco m e a n e sse ntia l to ol in r e se arc h , fo re nsic s, a nd d ia gnostic m ed ic in e. T he p ro ce ss in vo lv e st h re e m ain s te ps: d enatu ra tio n, a nnealin g, a nd e xte nsio n.D urin g th e d enatu ra tio n s te p, th e s a m ple is h eate d to a