Exam 1 Review Review Questions Histology And Its Methods Of Study 1.

Review QuestionsHistology & Its Methods of Study1. In preparing tissue for routine light microscopy, which procedure immediately precedes clearing the specimen with an organic solvent?a. Dehydrationb. Fixation: solution cross-links proteins and inactivates enzymes)c. Dehydration: tissue goes through series of increasingly concentrated alcohol solutions ending in 100%)d. Clearing: alcohol is removed in organic solventse. Infiltration: tissue is placed in melted paraffinf. Embedding: paraffin-infiltrated tissue is placed in small mold to hardeng. Trimming: paraffin block is trimmed to expose tissue for slicing2. Which of the following staining procedures relies on the cationic and anionic properties of the material to be stained?a. Hematoxylin & eosin (H&E)b. Periodic acid-Schiff (PAS): uses polysaccharides and carb-rich tissue and stains them purple/magenta3. In a light microscope for histology, resolution and magnification are largely dependent on which component?a. Objective lens4. Cellular storage deposits of glycogen, a free polysaccharide, could be best detected histologically using what procedure?a. Periodic acid-Schiff reaction5. Adding heavy metal compounds to the fixative and ultrathin sectioning of the embeddedtissue with a glass knife are techniques used for which histological procedure?a. Transmission electron microscopyb. SEM: surface of specimen is dried & sprayed with thin layer of heavy metal6. Resolution in electron microscopy greatly exceeds that of